FastQCFastQC Report
Sat 18 Jan 2025
SRR21995814_1.trimmed.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR21995814_1.trimmed.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16487711
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length31-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCGCTTCAGTCGGGTTCCCAGACCACAAGTATTCTAGGAAGCGGACAA4156272.5208289980337475No Hit
GCTGGTTATTTTGTTGCTGTTGATCGTTTTGCTGTTGGTTACCGTGTTGC3585752.17480158404038No Hit
GCGTGCGCTTCAGTCGGGTTCCCAGACCACAAGTATTCTAGGAAGCGGAC2775061.6831081039690714No Hit
CTCGAGAATACTACATGCTTAGATATCGTCTATTGTTTTAACACCAAGTG2457081.4902493135645087No Hit
GCGCTTCAGTCGGGTTCCCAGACCACAAGTATTCTAGGAAGCGGACAAGC2165651.3134934254973296No Hit
GCTCGAGAATACTACATGCTTAGATATCGTCTATTGTTTTAACACCAAGT1413170.8571050281024456No Hit
GCACTACGCTCGAGAATACTACATGCTTAGATATCGTCTATTGTTTTAAC1410260.8553400772247888No Hit
GTTGCTGGTTATTTTGTTGCTGTTGATCGTTTTGCTGTTGGTTACCGTGT1263090.7660796577523709No Hit
GTTATTTTGTTGCTGTTGATCGTTTTGCTGTTGGTTACCGTGTTGCTGTT1124270.6818836162278682No Hit
GTGTGCGTGCGCTTCAGTCGGGTTCCCAGACCACAAGTATTCTAGGAAGC1094370.6637488975880278No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGTGGTAATCTCGTAT1089260.6606496195863695TruSeq Adapter, Index 10 (97% over 36bp)
GCTGTTGTTGCTGGTTATTTTGTTGCTGTTGATCGTTTTGCTGTTGGTTA1086380.6589028640785856No Hit
GTGCGTGCGCTTCAGTCGGGTTCCCAGACCACAAGTATTCTAGGAAGCGG980440.5946489479346163No Hit
GCCGGGTTCTGTCGAGGATGGTCATTCCTCTAGGCGTACAATCACTCATA790520.4794601263935303No Hit
GGTTATTTTGTTGCTGTTGATCGTTTTGCTGTTGGTTACCGTGTTGCTGT779550.4728066861433949No Hit
GCTGTTGATCGTTTTGCTGTTGGTTACCGTGTTGCTGTTGATTACGGTTG770570.46736020542815193No Hit
CTGGTTATTTTGTTGCTGTTGATCGTTTTGCTGTTGGTTACCGTGTTGCT747730.4535074638316986No Hit
GGACCTTTTGAGGATGTTAAATATCATCCCACGCATCTTTAATCGCTTGT747390.4533012496398075No Hit
CGCTCGAGAATACTACATGCTTAGATATCGTCTATTGTTTTAACACCAAG728600.4419048829761754No Hit
GTTGCTGTTGATCGTTTTGCTGTTGGTTACCGTGTTGCTGTTGATTACGG588180.35673842172512604No Hit
TGGTTATTTTGTTGCTGTTGATCGTTTTGCTGTTGGTTACCGTGTTGCTG581360.352602007640721No Hit
TGCGCTTCAGTCGGGTTCCCAGACCACAAGTATTCTAGGAAGCGGACAAG566380.34351645295092814No Hit
GCCTTCCATATCTTCAAACTGAGTTCCTTCTTTAGATTCAGAACGAGCTC521810.3164841984433133No Hit
GCGGTTATCATTTTGCTGTTGTTGCTGGTTATTTTGTTGCTGTTGATCGT521560.3163325703610404No Hit
GGCGGGAGTTGAACCCGCGTCCGCCAGCACTACGCTCGAGAATACTACAT487870.2958991699939428No Hit
GGGGTTTGCCATGCCGTGAACTGTTACCAGACACGCGGTGCGCTCTTACC449690.2727425292692236No Hit
CCGGGTTCTGTCGAGGATGGTCATTCCTCTAGGCGTACAATCACTCATAC448080.271766044419386No Hit
GACCTTTTGAGGATGTTAAATATCATCCCACGCATCTTTAATCGCTTGTT447180.2712201833232035No Hit
GCGGGAGTTGAACCCGCGTCCGCCAGCACTACGCTCGAGAATACTACATG441890.26801173310230875No Hit
TGCGTGCGCTTCAGTCGGGTTCCCAGACCACAAGTATTCTAGGAAGCGGA437610.2654158603337965No Hit
TGCTGGTTATTTTGTTGCTGTTGATCGTTTTGCTGTTGGTTACCGTGTTG432790.2624924709075747No Hit
GTCGTCATCACGGCGGCTGCGACCACGACCTCGGCCACCTGAGCGACCGC431480.26169793975646466No Hit
GTGGGGTTTGCCATGCCGTGAACTGTTACCAGACACGCGGTGCGCTCTTA408650.24785126328330234No Hit
CGAGAATACTACATGCTTAGATATCGTCTATTGTTTTAACACCAAGTGAC400320.2427990155819689No Hit
CGCTTCAGTCGGGTTCCCAGACCACAAGTATTCTAGGAAGCGGACAAGCG399610.24236839182831382No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGTGGTAATCGCGTAT368830.22369994233887286TruSeq Adapter, Index 10 (97% over 36bp)
TGTTGATCGTTTTGCTGTTGGTTACCGTGTTGCTGTTGATTACGGTTGTC359890.21827772211679355No Hit
CTTCAGTCGGGTTCCCAGACCACAAGTATTCTAGGAAGCGGACAAGCGGC346760.21031421523582017No Hit
GCTGTTGGTTACCGTGTTGCTGTTGATTACGGTTGTCATTTTGCTGTTGT346280.21002308931785618No Hit
GTTGTTGCTGGTTATTTTGTTGCTGTTGATCGTTTTGCTGTTGGTTACCG336080.20383666356112137No Hit
GTCCGCCAGCACTACGCTCGAGAATACTACATGCTTAGATATCGTCTATT334530.20289656945102932No Hit
ACATGCTTAGATATCGTCTATTGTTTTAACACCAAGTGACCCGACGAACA331520.20107096734046345No Hit
GGGGCCTTGAATTGTAATTTGCCATTTTCGTTCGGCTGGCAAGTACCATT326240.19786858224285953No Hit
CTCTGCTGCACTTTCCGTCGGCTTTCACCGCCCAGGCGTTACCTGGCACC323330.19610363136520287No Hit
GCCGCGGTTATCATTTTGCTGTTGTTGCTGGTTATTTTGTTGCTGTTGAT321610.19506043015916522No Hit
CAGCACTACGCTCGAGAATACTACATGCTTAGATATCGTCTATTGTTTTA315190.1911666210063968No Hit
GCGCTTCTTGATAATAAGGTCGAGTAAGATAGCTTTCGCGCCAGCGAGAG304210.18450711563297054No Hit
CTACGCTCGAGAATACTACATGCTTAGATATCGTCTATTGTTTTAACACC303380.18400371039982447No Hit
CTGTTGGTTACCGTGTTGCTGTTGATTACGGTTGTCATTTTGCTGTTGTT302100.18322737461858715No Hit
CCCATTTTAGGCGGGACTCAGCTTTTAGCTCTTCCCATTTTTGCTGCAAG292490.1773987911360164No Hit
CTTAGATATCGTCTATTGTTTTAACACCAAGTGACCCGACGAACAGGGTA290850.1764041109163061No Hit
CGTGCGCTTCAGTCGGGTTCCCAGACCACAAGTATTCTAGGAAGCGGACA282030.17105467217371775No Hit
GTTGGTTGTTATTTGAGTTTTGTTGGCCAGAATCTTTTTGGTTAGATCCT280480.17011457806362568No Hit
GGCGCTTCAGTCGGGTTCCCAGACCACAAGTATTCTAGGAAGCGGACAAG276990.16799785003509582No Hit
GGTCGAGTAAGATAGCTTTCGCGCCAGCGAGAGTCAGAATAGTTATGATC271830.16486824641698294No Hit
GGTAGTTTTAATGTGATTTTTTCTTGCCGCCGCCATCTTGTTTATTGACG269310.163339835347672No Hit
CCCTCATATTTTATTGAATTTTATGCTTATATTTTGATTTAGGAAGGTGA268960.16312755603248988No Hit
GCTTCAGTCGGGTTCCCAGACCACAAGTATTCTAGGAAGCGGACAAGCGG256910.15581908246693552No Hit
GTTCCCATTTTAGGCGGGACTCAGCTTTTAGCTCTTCCCATTTTTGCTGC255790.15513978865835287No Hit
CTCATATTTTATTGAATTTTATGCTTATATTTTGATTTAGGAAGGTGAGG252280.15301093038324118No Hit
GATCGTTTTGCTGTTGGTTACCGTGTTGCTGTTGATTACGGTTGTCATTT247380.1500390199706921No Hit
GGCCGCGGTTATCATTTTGCTGTTGTTGCTGGTTATTTTGTTGCTGTTGA246250.14935366103881856No Hit
GCAGGGGCCTTGAATTGTAATTTGCCATTTTCGTTCGGCTGGCAAGTACC242870.14730364936648876No Hit
GCGGTGTTAGTTACAGCATCACCAGCTTTAGAGACATCTTGACCAAAACC234210.1420512525965551No Hit
GTTGGTTACCGTGTTGCTGTTGATTACGGTTGTCATTTTGCTGTTGTTGT230700.1399223943214434No Hit
GTCGAGTAAGATAGCTTTCGCGCCAGCGAGAGTCAGAATAGTTATGATCG229190.13900656070451503No Hit
CCAGCACTACGCTCGAGAATACTACATGCTTAGATATCGTCTATTGTTTT228670.13869117429338734No Hit
GCTGCACTTTCCGTCGGCTTTCACCGCCCAGGCGTTACCTGGCACCCTGC227780.1381513783204958No Hit
GCCCACCTTTACTGGCTATTTCTCTGACTCTTTCAGGATCCATACTTGCA224450.1361316922646206No Hit
GTTGATCGTTTTGCTGTTGGTTACCGTGTTGCTGTTGATTACGGTTGTCA223930.13581630585349294No Hit
CGCGCTTCAGTCGGGTTCCCAGACCACAAGTATTCTAGGAAGCGGACAAG223080.13530077037376503No Hit
CGCGTGCGCTTCAGTCGGGTTCCCAGACCACAAGTATTCTAGGAAGCGGA221820.13453656483910956No Hit
GGGCCGCACCTAGGATTCCTATTTGGTCTTGCTTCTGGTGGGGTTTGCCA218230.13235918557767054No Hit
CATGCTTAGATATCGTCTATTGTTTTAACACCAAGTGACCCGACGAACAG213020.12919925634310306No Hit
GTTGGCCGCGGTTATCATTTTGCTGTTGTTGCTGGTTATTTTGTTGCTGT206310.12512955861489808No Hit
TTGCTGGTTATTTTGTTGCTGTTGATCGTTTTGCTGTTGGTTACCGTGTT205170.1244381345597336No Hit
CTTGAATTGTAATTTGCCATTTTCGTTCGGCTGGCAAGTACCATTAAAGG204890.12426831110758794No Hit
GTGGAGATGGCGGGAGTTGAACCCGCGTCCGCCAGCACTACGCTCGAGAA202610.12288546299725898No Hit
CTTCCATATCTTCAAACTGAGTTCCTTCTTTAGATTCAGAACGAGCTCGA202240.12266105343549509No Hit
CGCGGTTATCATTTTGCTGTTGTTGCTGGTTATTTTGTTGCTGTTGATCG201100.12196962938033061No Hit
ACGGGCCGCACCTAGGATTCCTATTTGGTCTTGCTTCTGGTGGGGTTTGC193390.11729341932303397No Hit
GTAGTTAATTGCGCTTCTTGATAATAAGGTCGAGTAAGATAGCTTTCGCG189360.11484917463679464No Hit
CCGCGGTTATCATTTTGCTGTTGTTGCTGGTTATTTTGTTGCTGTTGATC189160.11472787217097632No Hit
GTCGTTTGCGACTATTTAAATGCAAATTTTATTTACGAGAGAAAATGCGC188650.11441855088313957No Hit
GGCCGCACCTAGGATTCCTATTTGGTCTTGCTTCTGGTGGGGTTTGCCAT183520.11130714263489941No Hit
GCTTAGATATCGTCTATTGTTTTAACACCAAGTGACCCGACGAACAGGGT182610.11075521641542602No Hit
GTGCGGTGTTAGTTACAGCATCACCAGCTTTAGAGACATCTTGACCAAAA178390.10819573438665925No Hit
GTCGTCATCATCACGACCGCGACCACGGCCACGACCGCCTGAGCGACCGC177060.10738907298896735No Hit
GTGTAGTTAATTGCGCTTCTTGATAATAAGGTCGAGTAAGATAGCTTTCG176460.10702516559151237No Hit
GTGCTTTCAGGGTATATAATTCTTTTTATCGCTTCCGTTCTAACATCTTA174730.10597589926218381No Hit
TCGAGAATACTACATGCTTAGATATCGTCTATTGTTTTAACACCAAGTGA173660.10532693107005574No Hit
GCACTTTCCGTCGGCTTTCACCGCCCAGGCGTTACCTGGCACCCTGCCCT173190.10504187027538267No Hit
GCCTCTTCAAGTGATTAATCACCAGTTGTAACTGCAGAACCTGACGAAAC172510.10462944189160035No Hit
CGCTTGTTTAGCGTGTTCCCATTTTAGGCGGGACTCAGCTTTTAGCTCTT170350.1033193752607624No Hit
GTAGTTTTAATGTGATTTTTTCTTGCCGCCGCCATCTTGTTTATTGACGG170220.10324052865798047No Hit
GCTATGGACCTTTTGAGGATGTTAAATATCATCCCACGCATCTTTAATCG169620.10287662126052549No Hit
GCCGCTGCATGTCGTTACCCTTGAACCCTAAAGTTCAAGGTGGAGGTGAC168670.10230043454788842No Hit
TCCCATTTTAGGCGGGACTCAGCTTTTAGCTCTTCCCATTTTTGCTGCAA168410.10214274134232461No Hit
GGTGGAGATGGCGGGAGTTGAACCCGCGTCCGCCAGCACTACGCTCGAGA167940.10185768054765151No Hit
CTGCACTTTCCGTCGGCTTTCACCGCCCAGGCGTTACCTGGCACCCTGCC165900.10062039539630455No Hit
GCTGTTGTTGTTGCTGCTGATTATTGCGTTGATTTGCTTGATTATTAGTA165610.10044450682086799No Hit
CGTCGTCATCACGGCGGCTGCGACCACGACCTCGGCCACCTGAGCGACCG165550.10040811608112248No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)