FastQCFastQC Report
Sat 18 Jan 2025
SRR21995814_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR21995814_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16916963
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCGCTTCAGTCGGGTTCCCAGACCACAAGTATTCTAGGAAGCGGACAA4249712.5120998373053127No Hit
GCTGGTTATTTTGTTGCTGTTGATCGTTTTGCTGTTGGTTACCGTGTTGC3640912.1522243679317614No Hit
GCGTGCGCTTCAGTCGGGTTCCCAGACCACAAGTATTCTAGGAAGCGGAC2833771.6751056321397642No Hit
CTCGAGAATACTACATGCTTAGATATCGTCTATTGTTTTAACACCAAGTG2522241.4909531929578612No Hit
GCGCTTCAGTCGGGTTCCCAGACCACAAGTATTCTAGGAAGCGGACAAGC2214171.3088460381452627No Hit
GCACTACGCTCGAGAATACTACATGCTTAGATATCGTCTATTGTTTTAAC1448320.8561347565753972No Hit
GCTCGAGAATACTACATGCTTAGATATCGTCTATTGTTTTAACACCAAGT1442020.852410683879843No Hit
GTTGCTGGTTATTTTGTTGCTGTTGATCGTTTTGCTGTTGGTTACCGTGT1283430.758664542802393No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGTGGTAATCTCGTAT1263840.74708445008717TruSeq Adapter, Index 10 (97% over 36bp)
GTTATTTTGTTGCTGTTGATCGTTTTGCTGTTGGTTACCGTGTTGCTGTT1146230.6775625152103247No Hit
GTGTGCGTGCGCTTCAGTCGGGTTCCCAGACCACAAGTATTCTAGGAAGC1118000.6608751227983416No Hit
GCTGTTGTTGCTGGTTATTTTGTTGCTGTTGATCGTTTTGCTGTTGGTTA1104670.652995457872669No Hit
GTGCGTGCGCTTCAGTCGGGTTCCCAGACCACAAGTATTCTAGGAAGCGG1001990.5922989841616371No Hit
GCCGGGTTCTGTCGAGGATGGTCATTCCTCTAGGCGTACAATCACTCATA805130.4759305792653209No Hit
GGTTATTTTGTTGCTGTTGATCGTTTTGCTGTTGGTTACCGTGTTGCTGT793620.4691267575628084No Hit
GCTGTTGATCGTTTTGCTGTTGGTTACCGTGTTGCTGTTGATTACGGTTG782270.46241751548431004No Hit
GGACCTTTTGAGGATGTTAAATATCATCCCACGCATCTTTAATCGCTTGT760670.44964926624241003No Hit
CTGGTTATTTTGTTGCTGTTGATCGTTTTGCTGTTGGTTACCGTGTTGCT760230.4493891722763713No Hit
CGCTCGAGAATACTACATGCTTAGATATCGTCTATTGTTTTAACACCAAG743880.43972431694743314No Hit
GTTGCTGTTGATCGTTTTGCTGTTGGTTACCGTGTTGCTGTTGATTACGG597070.3529416006880195No Hit
TGGTTATTTTGTTGCTGTTGATCGTTTTGCTGTTGGTTACCGTGTTGCTG590900.3492943739369767No Hit
TGCGCTTCAGTCGGGTTCCCAGACCACAAGTATTCTAGGAAGCGGACAAG579640.3426383328969863No Hit
GCCTTCCATATCTTCAAACTGAGTTCCTTCTTTAGATTCAGAACGAGCTC532250.3146250305093178No Hit
GCGGTTATCATTTTGCTGTTGTTGCTGGTTATTTTGTTGCTGTTGATCGT529460.3129757983155723No Hit
GGCGGGAGTTGAACCCGCGTCCGCCAGCACTACGCTCGAGAATACTACAT500000.295561325043981No Hit
GGGGTTTGCCATGCCGTGAACTGTTACCAGACACGCGGTGCGCTCTTACC459200.27144352092039215No Hit
GACCTTTTGAGGATGTTAAATATCATCCCACGCATCTTTAATCGCTTGTT456370.26977064382064325No Hit
CCGGGTTCTGTCGAGGATGGTCATTCCTCTAGGCGTACAATCACTCATAC456200.26967015297012825No Hit
GCGGGAGTTGAACCCGCGTCCGCCAGCACTACGCTCGAGAATACTACATG452780.26764851350682745No Hit
TGCGTGCGCTTCAGTCGGGTTCCCAGACCACAAGTATTCTAGGAAGCGGA447780.26469290025638764No Hit
GTCGTCATCACGGCGGCTGCGACCACGACCTCGGCCACCTGAGCGACCGC445470.2633274069346844No Hit
TGCTGGTTATTTTGTTGCTGTTGATCGTTTTGCTGTTGGTTACCGTGTTG438880.2594319086706048No Hit
GTGGGGTTTGCCATGCCGTGAACTGTTACCAGACACGCGGTGCGCTCTTA418640.2474675862328244No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGTGGTAATCGCGTAT417560.2468291737707294TruSeq Adapter, Index 10 (97% over 36bp)
CGAGAATACTACATGCTTAGATATCGTCTATTGTTTTAACACCAAGTGAC411830.24344204098572542No Hit
CGCTTCAGTCGGGTTCCCAGACCACAAGTATTCTAGGAAGCGGACAAGCG409720.2421947721940398No Hit
TGTTGATCGTTTTGCTGTTGGTTACCGTGTTGCTGTTGATTACGGTTGTC366400.2165873389922293No Hit
CTTCAGTCGGGTTCCCAGACCACAAGTATTCTAGGAAGCGGACAAGCGGC354130.20933426407564998No Hit
GCTGTTGGTTACCGTGTTGCTGTTGATTACGGTTGTCATTTTGCTGTTGT352460.2083470892500031No Hit
GTCCGCCAGCACTACGCTCGAGAATACTACATGCTTAGATATCGTCTATT342700.2025777321851446No Hit
GTTGTTGCTGGTTATTTTGTTGCTGTTGATCGTTTTGCTGTTGGTTACCG341690.20198069830855572No Hit
ACATGCTTAGATATCGTCTATTGTTTTAACACCAAGTGACCCGACGAACA337030.19922606675914584No Hit
CTCTGCTGCACTTTCCGTCGGCTTTCACCGCCCAGGCGTTACCTGGCACC334140.19751772230039163No Hit
GGGGCCTTGAATTGTAATTTGCCATTTTCGTTCGGCTGGCAAGTACCATT331940.19621725247019808No Hit
GCCGCGGTTATCATTTTGCTGTTGTTGCTGGTTATTTTGTTGCTGTTGAT327770.19375227101933132No Hit
CAGCACTACGCTCGAGAATACTACATGCTTAGATATCGTCTATTGTTTTA323710.1913523130599742No Hit
CTACGCTCGAGAATACTACATGCTTAGATATCGTCTATTGTTTTAACACC312040.18445391173344766No Hit
GCGCTTCTTGATAATAAGGTCGAGTAAGATAGCTTTCGCGCCAGCGAGAG311200.18395736870737378No Hit
CTGTTGGTTACCGTGTTGCTGTTGATTACGGTTGTCATTTTGCTGTTGTT307630.18184706084655974No Hit
CCCATTTTAGGCGGGACTCAGCTTTTAGCTCTTCCCATTTTTGCTGCAAG298860.17666291520528835No Hit
CTTAGATATCGTCTATTGTTTTAACACCAAGTGACCCGACGAACAGGGTA298650.17653877944876986No Hit
CGTGCGCTTCAGTCGGGTTCCCAGACCACAAGTATTCTAGGAAGCGGACA287350.1698590935027759No Hit
GTTGGTTGTTATTTGAGTTTTGTTGGCCAGAATCTTTTTGGTTAGATCCT284100.16793794488999No Hit
GGCGCTTCAGTCGGGTTCCCAGACCACAAGTATTCTAGGAAGCGGACAAG282950.16725815384238885No Hit
GGTCGAGTAAGATAGCTTTCGCGCCAGCGAGAGTCAGAATAGTTATGATC276720.16357545973234086No Hit
CCCTCATATTTTATTGAATTTTATGCTTATATTTTGATTTAGGAAGGTGA273700.1617902693290752No Hit
GGTAGTTTTAATGTGATTTTTTCTTGCCGCCGCCATCTTGTTTATTGACG273700.1617902693290752No Hit
GCTTCAGTCGGGTTCCCAGACCACAAGTATTCTAGGAAGCGGACAAGCGG262250.15502191498556805No Hit
GTTCCCATTTTAGGCGGGACTCAGCTTTTAGCTCTTCCCATTTTTGCTGC260320.15388104827089827No Hit
CTCATATTTTATTGAATTTTATGCTTATATTTTGATTTAGGAAGGTGAGG258080.15255693353470123No Hit
GATCGTTTTGCTGTTGGTTACCGTGTTGCTGTTGATTACGGTTGTCATTT252220.14909295480518578No Hit
GGCCGCGGTTATCATTTTGCTGTTGTTGCTGGTTATTTTGTTGCTGTTGA249720.14761514817996588No Hit
GCAGGGGCCTTGAATTGTAATTTGCCATTTTCGTTCGGCTGGCAAGTACC247000.14600729457172662No Hit
GCGGTGTTAGTTACAGCATCACCAGCTTTAGAGACATCTTGACCAAAACC240660.14225957697016894No Hit
GCTGCACTTTCCGTCGGCTTTCACCGCCCAGGCGTTACCTGGCACCCTGC236480.13978868429280125No Hit
GTTGGTTACCGTGTTGCTGTTGATTACGGTTGTCATTTTGCTGTTGTTGT236050.13953450155326344No Hit
GTCGAGTAAGATAGCTTTCGCGCCAGCGAGAGTCAGAATAGTTATGATCG235210.13903795852718953No Hit
CCAGCACTACGCTCGAGAATACTACATGCTTAGATATCGTCTATTGTTTT233710.13815127455205758No Hit
GCCCACCTTTACTGGCTATTTCTCTGACTCTTTCAGGATCCATACTTGCA229110.13543211036165298No Hit
GTTGATCGTTTTGCTGTTGGTTACCGTGTTGCTGTTGATTACGGTTGTCA228980.13535526441714155No Hit
CGCGCTTCAGTCGGGTTCCCAGACCACAAGTATTCTAGGAAGCGGACAAG228210.1349000999765738No Hit
CGCGTGCGCTTCAGTCGGGTTCCCAGACCACAAGTATTCTAGGAAGCGGA227540.13450404780101485No Hit
GGGCCGCACCTAGGATTCCTATTTGGTCTTGCTTCTGGTGGGGTTTGCCA222620.1315957243625821No Hit
CATGCTTAGATATCGTCTATTGTTTTAACACCAAGTGACCCGACGAACAG217510.12857508762063263No Hit
GTTGGCCGCGGTTATCATTTTGCTGTTGTTGCTGGTTATTTTGTTGCTGT209410.12378699415492012No Hit
CTTGAATTGTAATTTGCCATTTTCGTTCGGCTGGCAAGTACCATTAAAGG208930.12350325528287791No Hit
GTGGAGATGGCGGGAGTTGAACCCGCGTCCGCCAGCACTACGCTCGAGAA208580.1232963623553471No Hit
TTGCTGGTTATTTTGTTGCTGTTGATCGTTTTGCTGTTGGTTACCGTGTT208080.12300080103030313No Hit
CTTCCATATCTTCAAACTGAGTTCCTTCTTTAGATTCAGAACGAGCTCGA207550.12268750602575651No Hit
CGCGGTTATCATTTTGCTGTTGTTGCTGGTTATTTTGTTGCTGTTGATCG204470.1208668482634856No Hit
ACGGGCCGCACCTAGGATTCCTATTTGGTCTTGCTTCTGGTGGGGTTTGC196390.11609057725077486No Hit
GTCGTTTGCGACTATTTAAATGCAAATTTTATTTACGAGAGAAAATGCGC194500.11497335544210861No Hit
GTAGTTAATTGCGCTTCTTGATAATAAGGTCGAGTAAGATAGCTTTCGCG192870.11400982552246523No Hit
CCGCGGTTATCATTTTGCTGTTGTTGCTGGTTATTTTGTTGCTGTTGATC192840.11399209184296259No Hit
GGCCGCACCTAGGATTCCTATTTGGTCTTGCTTCTGGTGGGGTTTGCCAT187200.11065816009646649No Hit
GCTTAGATATCGTCTATTGTTTTAACACCAAGTGACCCGACGAACAGGGT187130.11061678151096033No Hit
GTGCGGTGTTAGTTACAGCATCACCAGCTTTAGAGACATCTTGACCAAAA183490.10846509506464014No Hit
GTCGTCATCATCACGACCGCGACCACGGCCACGACCGCCTGAGCGACCGC182100.1076434345810179No Hit
GCACTTTCCGTCGGCTTTCACCGCCCAGGCGTTACCTGGCACCCTGCCCT179140.10589371153675751No Hit
GTGTAGTTAATTGCGCTTCTTGATAATAAGGTCGAGTAAGATAGCTTTCG179110.10587597785725487No Hit
GTGCTTTCAGGGTATATAATTCTTTTTATCGCTTCCGTTCTAACATCTTA178720.10564544002372056No Hit
TCGAGAATACTACATGCTTAGATATCGTCTATTGTTTTAACACCAAGTGA178060.10525529907466251No Hit
GCCTCTTCAAGTGATTAATCACCAGTTGTAACTGCAGAACCTGACGAAAC176660.10442772736453936No Hit
GTAGTTTTAATGTGATTTTTTCTTGCCGCCGCCATCTTGTTTATTGACGG173820.10274893903828956No Hit
CGCTTGTTTAGCGTGTTCCCATTTTAGGCGGGACTCAGCTTTTAGCTCTT173760.10271347167928428No Hit
GCTATGGACCTTTTGAGGATGTTAAATATCATCCCACGCATCTTTAATCG172660.1020632367641875No Hit
GCCGCTGCATGTCGTTACCCTTGAACCCTAAAGTTCAAGGTGGAGGTGAC172440.10193318978116817No Hit
CTGCACTTTCCGTCGGCTTTCACCGCCCAGGCGTTACCTGGCACCCTGCC172180.1017794978921453No Hit
TCCCATTTTAGGCGGGACTCAGCTTTTAGCTCTTCCCATTTTTGCTGCAA171820.10156669373811364No Hit
GGTGGAGATGGCGGGAGTTGAACCCGCGTCCGCCAGCACTACGCTCGAGA171170.10118246401555646No Hit
CGTCGTCATCACGGCGGCTGCGACCACGACCTCGGCCACCTGAGCGACCG171150.1011706415625547No Hit
GCTGTTGTTGTTGCTGCTGATTATTGCGTTGATTTGCTTGATTATTAGTA170380.10071547712198697No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)