FastQCFastQC Report
Sat 18 Jan 2025
SRR21995814_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR21995814_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16916963
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAACTCATAGATGCATGCCGAGAGCGCATTTTCTCTCGTAAATAAAATT3227611.907913376650407No Hit
GATGAAACTCATAGATGCATGCCGAGAGCGCATTTTCTCTCGTAAATAAA1621930.9587595598571683No Hit
AACTCATAGATGCATGCCGAGAGCGCATTTTCTCTCGTAAATAAAATTTG1275130.753758224806663No Hit
CTCAGATAAAACACATCGTCATAATTGATATGAGGTATTAAAAATGAGCA1185090.7005335413927429No Hit
CTCATAGATGCATGCCGAGAGCGCATTTTCTCTCGTAAATAAAATTTGCA1154960.6827230159455926No Hit
TGAAACTCATAGATGCATGCCGAGAGCGCATTTTCTCTCGTAAATAAAAT1053050.6224817066751284No Hit
AAAACACATCGTCATAATTGATATGAGGTATTAAAAATGAGCACTACTAA958920.5668393316223486No Hit
GATAAAACACATCGTCATAATTGATATGAGGTATTAAAAATGAGCACTAC821820.4857964162952889No Hit
GTTATTGGCTTCGACGCCGGTGATGAAACTCATAGATGCATGCCGAGAGC805860.47636209879988506No Hit
ATTGGCTTCGACGCCGGTGATGAAACTCATAGATGCATGCCGAGAGCGCA795150.470031175217443No Hit
AACACATCGTCATAATTGATATGAGGTATTAAAAATGAGCACTACTAATA741350.4382287766427106No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG691850.4089682054633565No Hit
ATGAAACTCATAGATGCATGCCGAGAGCGCATTTTCTCTCGTAAATAAAA652510.3857134404088961No Hit
AATTGATATGAGGTATTAAAAATGAGCACTACTAATAATCAAGCAAATCA620090.36654924409304435No Hit
GCTGGTTATTTTGTTGCTGTTGATCGTTTTGCTGTTGGTTACCGTGTTGC580930.34340088111559974No Hit
ATAAAACACATCGTCATAATTGATATGAGGTATTAAAAATGAGCACTACT576350.3406935393781969No Hit
CCGGTGATGAAACTCATAGATGCATGCCGAGAGCGCATTTTCTCTCGTAA536500.3171373017721916No Hit
CGCCGGTGATGAAACTCATAGATGCATGCCGAGAGCGCATTTTCTCTCGT528590.31246152160999585No Hit
GAGGTATTAAAAATGAGCACTACTAATAATCAAGCAAATCAACGCAATAA507410.2999415438811328No Hit
GACGAAACTTACGCTCTAGCTGCCTAAGGGCCGCTTGTCCGCTTCCTAGA503470.2976125206397862No Hit
CGGATGGTCGCTGCTGTGGAGGTCTCCGTGACTGAAGCAGGAGAGGAAAG500560.2958923537280303No Hit
AGCAAATCAACGCAATAATCAGCAGCAACAACAACAGCAAAATGACAACC461820.2729922622636226No Hit
TATTGGCTTCGACGCCGGTGATGAAACTCATAGATGCATGCCGAGAGCGC461370.27272625707108306No Hit
AGATAAAACACATCGTCATAATTGATATGAGGTATTAAAAATGAGCACTA420490.24856116313548715No Hit
TCTCAGATAAAACACATCGTCATAATTGATATGAGGTATTAAAAATGAGC417600.24685281867673292No Hit
ATTAAAAATGAGCACTACTAATAATCAAGCAAATCAACGCAATAATCAGC417450.24676415027921972No Hit
CGGTGATGAAACTCATAGATGCATGCCGAGAGCGCATTTTCTCTCGTAAA413080.24418094429833537No Hit
CCGTAATCAACAGCAACACGGTAACCAACAGCAAAACGATCAACAGCAAC409280.24193467822800108No Hit
CATAGATGCATGCCGAGAGCGCATTTTCTCTCGTAAATAAAATTTGCATT392640.23209839733053741No Hit
GGATGGTCGCTGCTGTGGAGGTCTCCGTGACTGAAGCAGGAGAGGAAAGT386340.22837432463498325No Hit
ATTGATATGAGGTATTAAAAATGAGCACTACTAATAATCAAGCAAATCAA383010.2264058862101903No Hit
CTTACGCTCTAGCTGCCTAAGGGCCGCTTGTCCGCTTCCTAGAATACTTG367070.2169833911677882No Hit
AAACTCATAGATGCATGCCGAGAGCGCATTTTCTCTCGTAAATAAAATTT358870.21213618543706692No Hit
CACATCGTCATAATTGATATGAGGTATTAAAAATGAGCACTACTAATAAT351210.20760818593739314No Hit
AAAATGAGCACTACTAATAATCAAGCAAATCAACGCAATAATCAGCAGCA348970.2062840712011961No Hit
TTAAAAATGAGCACTACTAATAATCAAGCAAATCAACGCAATAATCAGCA328190.19400054253236826No Hit
CTTGTGGTCTGGGAACCCGACTGAAGCGCACGCACACAAGTCCGTATAGA325860.1926232267576633No Hit
TAAAAATGAGCACTACTAATAATCAAGCAAATCAACGCAATAATCAGCAG321550.19007548813578418No Hit
ACGAAACTTACGCTCTAGCTGCCTAAGGGCCGCTTGTCCGCTTCCTAGAA321300.1899277074732622No Hit
CTAATAATCAAGCAAATCAACGCAATAATCAGCAGCAACAACAACAGCAA318770.18843216716853967No Hit
GCCGAGAGCGCATTTTCTCTCGTAAATAAAATTTGCATTTAAATAGTCGC318510.18827847527951677No Hit
CAACAACAGCAAAATGACAACCGTAATCAACAGCAACACGGTAACCAACA312970.18500365579802946No Hit
CGCAATAATCAGCAGCAACAACAACAGCAAAATGACAACCGTAATCAACA289410.17107680616195708No Hit
GGTCGCTGCTGTGGAGGTCTCCGTGACTGAAGCAGGAGAGGAAAGTCCGG288550.17056844068288143No Hit
AAACACATCGTCATAATTGATATGAGGTATTAAAAATGAGCACTACTAAT285670.16886600745062813No Hit
CCCGACTGAAGCGCACGCACACAAGTCCGTATAGAGTCAAGCCTCGGGGC281040.16612910958072083No Hit
GCCGGATGGTCGCTGCTGTGGAGGTCTCCGTGACTGAAGCAGGAGAGGAA277620.16410747011742No Hit
GCAAAATGACAACCGTAATCAACAGCAACACGGTAACCAACAGCAAAACG274270.16212720923962534No Hit
CGTCATAATTGATATGAGGTATTAAAAATGAGCACTACTAATAATCAAGC270370.15982183090428229No Hit
GTCAAGCCTCGGGGCTTTATACCAAACTTAGAGGATCGCACCTTGTACCC266930.15778836898797968No Hit
AATCAAGCAAATCAACGCAATAATCAGCAGCAACAACAACAGCAAAATGA262670.155270186498605No Hit
GAAACTTACGCTCTAGCTGCCTAAGGGCCGCTTGTCCGCTTCCTAGAATA254620.1505116491653969No Hit
GTCATAATTGATATGAGGTATTAAAAATGAGCACTACTAATAATCAAGCA253550.14987914792980278No Hit
GTTATTTTGTTGCTGTTGATCGTTTTGCTGTTGGTTACCGTGTTGCTGTT252630.14933531509172185No Hit
CAGATAAAACACATCGTCATAATTGATATGAGGTATTAAAAATGAGCACT247230.14614325278124685No Hit
CAACAGCAAAATGACAACCGTAATCAACAGCAACACGGTAACCAACAGCA242660.14344182227034485No Hit
GGGAATTCTGATGACAGATCAAAATACAGATCATGCTCAATTGGTAGCTG241920.14300439150927977No Hit
TAATAATCAAGCAAATCAACGCAATAATCAGCAGCAACAACAACAGCAAA241740.14289798943226392No Hit
TGACAACCGTAATCAACAGCAACACGGTAACCAACAGCAAAACGATCAAC239280.14144382771304756No Hit
AAACGACGAAACTTACGCTCTAGCTGCCTAAGGGCCGCTTGTCCGCTTCC235130.1389906687151825No Hit
ACTCATAGATGCATGCCGAGAGCGCATTTTCTCTCGTAAATAAAATTTGC234580.13866555125763413No Hit
GCAGCAACAACAACAGCAAAATGACAACCGTAATCAACAGCAACACGGTA234090.13837590115909104No Hit
GGGAACCCGACTGAAGCGCACGCACACAAGTCCGTATAGAGTCAAGCCTC232660.13753059576946525No Hit
ATTCTGATGACAGATCAAAATACAGATCATGCTCAATTGGTAGCTGGCGA231510.1368508047218641No Hit
GCGCATTTTCTCTCGTAAATAAAATTTGCATTTAAATAGTCGCAAACGAC231110.1366143556618289No Hit
GCAATAATCAGCAGCAACAACAACAGCAAAATGACAACCGTAATCAACAG230750.13640155150779723No Hit
TTGGCTTCGACGCCGGTGATGAAACTCATAGATGCATGCCGAGAGCGCAT229270.13552668998566705No Hit
GCTGTTGTTGCTGGTTATTTTGTTGCTGTTGATCGTTTTGCTGTTGGTTA219220.12958590735228304No Hit
CCGTGACTGAAGCAGGAGAGGAAAGTCCGGGCTTCATAGGGCAGGGTGCC213120.12598005918674646No Hit
CAATAATCAGCAGCAACAACAACAGCAAAATGACAACCGTAATCAACAGC213000.1259091244687359No Hit
GTTGCTGGTTATTTTGTTGCTGTTGATCGTTTTGCTGTTGGTTACCGTGT212420.12556627333168488No Hit
GTCTCCGTGACTGAAGCAGGAGAGGAAAGTCCGGGCTTCATAGGGCAGGG209780.12400570953545267No Hit
CATCGTCATAATTGATATGAGGTATTAAAAATGAGCACTACTAATAATCA207720.12278799687627147No Hit
GCTCTAGCTGCCTAAGGGCCGCTTGTCCGCTTCCTAGAATACTTGTGGTC206250.12191904658064216No Hit
CAAACGACGAAACTTACGCTCTAGCTGCCTAAGGGCCGCTTGTCCGCTTC205710.12159984034959466No Hit
ATAGAGTCAAGCCTCGGGGCTTTATACCAAACTTAGAGGATCGCACCTTG204500.12088458194298823No Hit
TCGCAAACGACGAAACTTACGCTCTAGCTGCCTAAGGGCCGCTTGTCCGC203000.11999789796785629No Hit
GGTGATGAAACTCATAGATGCATGCCGAGAGCGCATTTTCTCTCGTAAAT202600.11976144890782109No Hit
GCACCTTGTACCCTGTTCGTCGGGTCACTTGGTGTTAAAACAATAGACGA202430.11966095805730616No Hit
TGATGAAACTCATAGATGCATGCCGAGAGCGCATTTTCTCTCGTAAATAA198560.11737331340146574No Hit
ATTTTCTCTCGTAAATAAAATTTGCATTTAAATAGTCGCAAACGACGAAA198380.1172669113244499No Hit
AGAATACTTGTGGTCTGGGAACCCGACTGAAGCGCACGCACACAAGTCCG194710.1150974911986271No Hit
GCCGGTGATGAAACTCATAGATGCATGCCGAGAGCGCATTTTCTCTCGTA193800.11455956958704704No Hit
GCAACAACAACAGCAAAATGACAACCGTAATCAACAGCAACACGGTAACC193400.11432312052701186No Hit
GAGGTCTCCGTGACTGAAGCAGGAGAGGAAAGTCCGGGCTTCATAGGGCA191080.11295171597880778No Hit
ATCAGCAGCAACAACAACAGCAAAATGACAACCGTAATCAACAGCAACAC190650.11269753323926997No Hit
TCATAATTGATATGAGGTATTAAAAATGAGCACTACTAATAATCAAGCAA188700.11154484407159843No Hit
GGAACACAAAATGAAAACGACCATGTTGAAAATCTTAACAGCATCTCTAA184940.10932222290726769No Hit
CAACAACAACAGCAAAATGACAACCGTAATCAACAGCAACACGGTAACCA180050.10643163314833755No Hit
GGCCGCTTGTCCGCTTCCTAGAATACTTGTGGTCTGGGAACCCGACTGAA179790.1062779412593147No Hit
AAAAATGAGCACTACTAATAATCAAGCAAATCAACGCAATAATCAGCAGC178710.1056395287972197No Hit
GATGGTCGCTGCTGTGGAGGTCTCCGTGACTGAAGCAGGAGAGGAAAGTC176350.1042444793430121No Hit
AGTCAAGCCTCGGGGCTTTATACCAAACTTAGAGGATCGCACCTTGTACC174650.10323957083786257No Hit
CGCTGCTGTGGAGGTCTCCGTGACTGAAGCAGGAGAGGAAAGTCCGGGCT174160.10294992073931945No Hit
GAATACTTGTGGTCTGGGAACCCGACTGAAGCGCACGCACACAAGTCCGT174040.10287898602130892No Hit
CGAAACTTACGCTCTAGCTGCCTAAGGGCCGCTTGTCCGCTTCCTAGAAT173960.10283169620930188No Hit
AATCAACGCAATAATCAGCAGCAACAACAACAGCAAAATGACAACCGTAA172860.1021814612942051No Hit
CTAAAACAACGGAAGTGAGTAAAACGAGAAGAAGGATGTGGAAAGCATCT171940.10163762845612417No Hit
GCCGCTTGTCCGCTTCCTAGAATACTTGTGGTCTGGGAACCCGACTGAAG170060.10052631787395881No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)