FastQCFastQC Report
Sat 18 Jan 2025
SRR21995814_2.trimmed.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR21995814_2.trimmed.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16487711
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length31-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAACTCATAGATGCATGCCGAGAGCGCATTTTCTCTCGTAAATAAAATT3227161.957312327951406No Hit
GATGAAACTCATAGATGCATGCCGAGAGCGCATTTTCTCTCGTAAATAAA1621700.9835810440879271No Hit
AACTCATAGATGCATGCCGAGAGCGCATTTTCTCTCGTAAATAAAATTTG1274890.7732365032356523No Hit
CTCAGATAAAACACATCGTCATAATTGATATGAGGTATTAAAAATGAGCA1184900.7186564587406948No Hit
CTCATAGATGCATGCCGAGAGCGCATTTTCTCTCGTAAATAAAATTTGCA1154740.7003640468952907No Hit
TGAAACTCATAGATGCATGCCGAGAGCGCATTTTCTCTCGTAAATAAAAT1052840.6385604405608517No Hit
AAAACACATCGTCATAATTGATATGAGGTATTAAAAATGAGCACTACTAA958690.5814573047768729No Hit
GATAAAACACATCGTCATAATTGATATGAGGTATTAAAAATGAGCACTAC821680.4983590505680261No Hit
GTTATTGGCTTCGACGCCGGTGATGAAACTCATAGATGCATGCCGAGAGC805760.488703374288887No Hit
ATTGGCTTCGACGCCGGTGATGAAACTCATAGATGCATGCCGAGAGCGCA793940.48153439855902375No Hit
AACACATCGTCATAATTGATATGAGGTATTAAAAATGAGCACTACTAATA741160.44952267782956656No Hit
ATGAAACTCATAGATGCATGCCGAGAGCGCATTTTCTCTCGTAAATAAAA652440.39571290399255543No Hit
AATTGATATGAGGTATTAAAAATGAGCACTACTAATAATCAAGCAAATCA619980.37602551379024046No Hit
GCTGGTTATTTTGTTGCTGTTGATCGTTTTGCTGTTGGTTACCGTGTTGC580840.35228662122959337No Hit
ATAAAACACATCGTCATAATTGATATGAGGTATTAAAAATGAGCACTACT576150.3494420784061535No Hit
CCGGTGATGAAACTCATAGATGCATGCCGAGAGCGCATTTTCTCTCGTAA536390.3253271482014696No Hit
CGCCGGTGATGAAACTCATAGATGCATGCCGAGAGCGCATTTTCTCTCGT528500.32054176592493644No Hit
GAGGTATTAAAAATGAGCACTACTAATAATCAAGCAAATCAACGCAATAA507240.307647313808448No Hit
GACGAAACTTACGCTCTAGCTGCCTAAGGGCCGCTTGTCCGCTTCCTAGA503240.3052212644920814No Hit
CGGATGGTCGCTGCTGTGGAGGTCTCCGTGACTGAAGCAGGAGAGGAAAG500480.3035472904637885No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG480480.2914170438819555No Hit
AGCAAATCAACGCAATAATCAGCAGCAACAACAACAGCAAAATGACAACC461610.27997215623199606No Hit
TATTGGCTTCGACGCCGGTGATGAAACTCATAGATGCATGCCGAGAGCGC461340.2798083979031413No Hit
AGATAAAACACATCGTCATAATTGATATGAGGTATTAAAAATGAGCACTA420390.2549717180268383No Hit
TCTCAGATAAAACACATCGTCATAATTGATATGAGGTATTAAAAATGAGC417530.25323709276563616No Hit
ATTAAAAATGAGCACTACTAATAATCAAGCAAATCAACGCAATAATCAGC417310.25310366005323603No Hit
CGGTGATGAAACTCATAGATGCATGCCGAGAGCGCATTTTCTCTCGTAAA413040.25051385240801466No Hit
CCGTAATCAACAGCAACACGGTAACCAACAGCAAAACGATCAACAGCAAC409010.2480696077217753No Hit
CATAGATGCATGCCGAGAGCGCATTTTCTCTCGTAAATAAAATTTGCATT392600.23811674040138137No Hit
GGATGGTCGCTGCTGTGGAGGTCTCCGTGACTGAAGCAGGAGAGGAAAGT386310.2343017778513949No Hit
ATTGATATGAGGTATTAAAAATGAGCACTACTAATAATCAAGCAAATCAA382940.23225783130235603No Hit
CTTACGCTCTAGCTGCCTAAGGGCCGCTTGTCCGCTTCCTAGAATACTTG367030.22260822014650788No Hit
AAACTCATAGATGCATGCCGAGAGCGCATTTTCTCTCGTAAATAAAATTT358790.21761055855479272No Hit
CACATCGTCATAATTGATATGAGGTATTAAAAATGAGCACTACTAATAAT351100.21294647874407793No Hit
AAAATGAGCACTACTAATAATCAAGCAAATCAACGCAATAATCAGCAGCA348660.2114665886610943No Hit
TTAAAAATGAGCACTACTAATAATCAAGCAAATCAACGCAATAATCAGCA327290.19850542018840575No Hit
CTTGTGGTCTGGGAACCCGACTGAAGCGCACGCACACAAGTCCGTATAGA325740.19756532607831373No Hit
ACGAAACTTACGCTCTAGCTGCCTAAGGGCCGCTTGTCCGCTTCCTAGAA321230.1948299554741104No Hit
TAAAAATGAGCACTACTAATAATCAAGCAAATCAACGCAATAATCAGCAG321150.19478143448778304No Hit
CTAATAATCAAGCAAATCAACGCAATAATCAGCAGCAACAACAACAGCAA318570.1932166326787266No Hit
GCCGAGAGCGCATTTTCTCTCGTAAATAAAATTTGCATTTAAATAGTCGC318480.19316204656910835No Hit
CAACAACAGCAAAATGACAACCGTAATCAACAGCAACACGGTAACCAACA311380.18885580903255764No Hit
CGCAATAATCAGCAGCAACAACAACAGCAAAATGACAACCGTAATCAACA288830.17517895601154096No Hit
GGTCGCTGCTGTGGAGGTCTCCGTGACTGAAGCAGGAGAGGAAAGTCCGG288510.17498487206623164No Hit
AAACACATCGTCATAATTGATATGAGGTATTAAAAATGAGCACTACTAAT285620.17323205143515677No Hit
CCCGACTGAAGCGCACGCACACAAGTCCGTATAGAGTCAAGCCTCGGGGC281000.17042996447475334No Hit
GCCGGATGGTCGCTGCTGTGGAGGTCTCCGTGACTGAAGCAGGAGAGGAA277580.1683556923092599No Hit
GCAAAATGACAACCGTAATCAACAGCAACACGGTAACCAACAGCAAAACG274140.16626928989718462No Hit
CGTCATAATTGATATGAGGTATTAAAAATGAGCACTACTAATAATCAAGC270280.16392815230689087No Hit
GTCAAGCCTCGGGGCTTTATACCAAACTTAGAGGATCGCACCTTGTACCC266900.1618781406345611No Hit
AATCAAGCAAATCAACGCAATAATCAGCAGCAACAACAACAGCAAAATGA262480.159197356139976No Hit
GAAACTTACGCTCTAGCTGCCTAAGGGCCGCTTGTCCGCTTCCTAGAATA254570.15439984361686107No Hit
GTCATAATTGATATGAGGTATTAAAAATGAGCACTACTAATAATCAAGCA253460.15372661493156933No Hit
GTTATTTTGTTGCTGTTGATCGTTTTGCTGTTGGTTACCGTGTTGCTGTT252590.15319894920525962No Hit
CAGATAAAACACATCGTCATAATTGATATGAGGTATTAAAAATGAGCACT247180.1499177175048738No Hit
GGGAATTCTGATGACAGATCAAAATACAGATCATGCTCAATTGGTAGCTG241910.1467213975305608No Hit
CAACAGCAAAATGACAACCGTAATCAACAGCAACACGGTAACCAACAGCA241670.1465758345715788No Hit
TAATAATCAAGCAAATCAACGCAATAATCAGCAGCAACAACAACAGCAAA241590.14652731358525145No Hit
TGACAACCGTAATCAACAGCAACACGGTAACCAACAGCAAAACGATCAAC239120.14502922813239508No Hit
AAACGACGAAACTTACGCTCTAGCTGCCTAAGGGCCGCTTGTCCGCTTCC235080.14257891832286482No Hit
ACTCATAGATGCATGCCGAGAGCGCATTTTCTCTCGTAAATAAAATTTGC234540.14225140166515535No Hit
GCAGCAACAACAACAGCAAAATGACAACCGTAATCAACAGCAACACGGTA233960.1418996245142822No Hit
GGGAACCCGACTGAAGCGCACGCACACAAGTCCGTATAGAGTCAAGCCTC232630.1410929631165903No Hit
ATTCTGATGACAGATCAAAATACAGATCATGCTCAATTGGTAGCTGGCGA231380.1403348227052257No Hit
GCGCATTTTCTCTCGTAAATAAAATTTGCATTTAAATAGTCGCAAACGAC231090.14015893412978916No Hit
GCAATAATCAGCAGCAACAACAACAGCAAAATGACAACCGTAATCAACAG230250.13964946377335216No Hit
TTGGCTTCGACGCCGGTGATGAAACTCATAGATGCATGCCGAGAGCGCAT229240.13903688632096958No Hit
GCTGTTGTTGCTGGTTATTTTGTTGCTGTTGATCGTTTTGCTGTTGGTTA218730.13266244174221636No Hit
CCGTGACTGAAGCAGGAGAGGAAAGTCCGGGCTTCATAGGGCAGGGTGCC213070.12922958195955764No Hit
CAATAATCAGCAGCAACAACAACAGCAAAATGACAACCGTAATCAACAGC212850.12909614924715748No Hit
GTTGCTGGTTATTTTGTTGCTGTTGATCGTTTTGCTGTTGGTTACCGTGT212350.12879289308261166No Hit
GTCTCCGTGACTGAAGCAGGAGAGGAAAGTCCGGGCTTCATAGGGCAGGG209770.1272280912735552No Hit
CATCGTCATAATTGATATGAGGTATTAAAAATGAGCACTACTAATAATCA207670.12595441538246274No Hit
GCTCTAGCTGCCTAAGGGCCGCTTGTCCGCTTCCTAGAATACTTGTGGTC206250.1250931678751526No Hit
CAAACGACGAAACTTACGCTCTAGCTGCCTAAGGGCCGCTTGTCCGCTTC205700.12475958609415219No Hit
ATAGAGTCAAGCCTCGGGGCTTTATACCAAACTTAGAGGATCGCACCTTG204500.12403177129924221No Hit
TCGCAAACGACGAAACTTACGCTCTAGCTGCCTAAGGGCCGCTTGTCCGC202990.12311593768231381No Hit
GGTGATGAAACTCATAGATGCATGCCGAGAGCGCATTTTCTCTCGTAAAT202530.12283694201093165No Hit
GCACCTTGTACCCTGTTCGTCGGGTCACTTGGTGTTAAAACAATAGACGA201620.12228501579145826No Hit
TGATGAAACTCATAGATGCATGCCGAGAGCGCATTTTCTCTCGTAAATAA198540.12041695781785598No Hit
ATTTTCTCTCGTAAATAAAATTTGCATTTAAATAGTCGCAAACGACGAAA198360.12030778559861947No Hit
AGAATACTTGTGGTCTGGGAACCCGACTGAAGCGCACGCACACAAGTCCG194690.11808188535085314No Hit
GCCGGTGATGAAACTCATAGATGCATGCCGAGAGCGCATTTTCTCTCGTA193770.1175238940080888No Hit
GCAACAACAACAGCAAAATGACAACCGTAATCAACAGCAACACGGTAACC193300.11723883321341573No Hit
ATCAGCAGCAACAACAACAGCAAAATGACAACCGTAATCAACAGCAACAC190530.11555879406183187No Hit
GAGGTCTCCGTGACTGAAGCAGGAGAGGAAAGTCCGGGCTTCATAGGGCA190290.11541323110284987No Hit
TCATAATTGATATGAGGTATTAAAAATGAGCACTACTAATAATCAAGCAA188660.11442461600643049No Hit
GGAACACAAAATGAAAACGACCATGTTGAAAATCTTAACAGCATCTCTAA184870.11212593427917313No Hit
CAACAACAACAGCAAAATGACAACCGTAATCAACAGCAACACGGTAACCA179890.10910550288029672No Hit
GGCCGCTTGTCCGCTTCCTAGAATACTTGTGGTCTGGGAACCCGACTGAA179760.10902665627751482No Hit
AAAAATGAGCACTACTAATAATCAAGCAAATCAACGCAATAATCAGCAGC178670.1083655578388049No Hit
GATGGTCGCTGCTGTGGAGGTCTCCGTGACTGAAGCAGGAGAGGAAAGTC176350.10695844923531228No Hit
AGTCAAGCCTCGGGGCTTTATACCAAACTTAGAGGATCGCACCTTGTACC174580.10588492241282008No Hit
CGCTGCTGTGGAGGTCTCCGTGACTGAAGCAGGAGAGGAAAGTCCGGGCT174120.10560592674143791No Hit
GAATACTTGTGGTCTGGGAACCCGACTGAAGCGCACGCACACAAGTCCGT174040.10555740575511056No Hit
CGAAACTTACGCTCTAGCTGCCTAAGGGCCGCTTGTCCGCTTCCTAGAAT173920.1054846242756196No Hit
AATCAACGCAATAATCAGCAGCAACAACAACAGCAAAATGACAACCGTAA172770.10478713509716418No Hit
CTAAAACAACGGAAGTGAGTAAAACGAGAAGAAGGATGTGGAAAGCATCT171810.1042048832612362No Hit
GCCGCTTGTCCGCTTCCTAGAATACTTGTGGTCTGGGAACCCGACTGAAG170040.10313135643874398No Hit
GGTTATTTTGTTGCTGTTGATCGTTTTGCTGTTGGTTACCGTGTTGCTGT168930.10245812775345225No Hit
GCCTAAGGGCCGCTTGTCCGCTTCCTAGAATACTTGTGGTCTGGGAACCC168920.10245206263016134No Hit
GTGATGAAACTCATAGATGCATGCCGAGAGCGCATTTTCTCTCGTAAATA165840.10058400465655906No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)